Production of Recombinant Soluble ABeta Oligomers with Split-luciferase Tags
Soluble oligomers of ABeta peptide are cytotoxic and play a central role in Alzheimer’s Disease, however, their mechanism of action remains unknown. We have made ABeta in bacteria and purified ABeta oligomers (ABOs) in order that we might describe their structure and oligomerization pathway. Purified, disease-relevant ABOs would also enable in vitro testing of proposed cytotoxic mechanisms. We have verifed that our soluble ABOs are similar in size and shape to patient-derived ABOs. Once we prove that our ABOs are cytotoxic, we will begin to study them using structural and biochemical techniques.